Brusatol-Enriched Brucea javanica Oil Ameliorated Dextran Sulfate Sodium-Induced Colitis in Mice: Involvement of NF-?B and RhoA/ROCK Signaling Pathways

Brucea javanica oil (BJO) is beneficial for the treatment of ulcerative colitis (UC), and that quassinoids in particular brusatol are bioactive components. However, it still uncertain whether or not other components BJO, such as oleic acid fatty acids, have an anti-UC effect. The present study aimed at comparing effects between brusatol-enriched BJO (BE-BJO) brusatol-free (BF-BJO) exploring mechanisms BE-BJO on colon inflammation intestinal epithelial barrier function. Balb/C mice received 3% (wt/vol) DSS one week to establish UC model. Different doses BE-BJO, BF-BJO, were treated. result illustrated alleviated DSS-induced loss body weight, increase disease activity index (DAI), a shortening colon, whereas BF-BJO did these protective effects. improved morphology tissue, inhibited production release TNF-?, IFN-?, IL-6, IL-1? reversed decreased expressions ZO-1, occludin, claudin-1, E-cadherin induced by but augmented claudin-2 expression. Mechanistically, repressed phosphorylation NF-?B subunit p65, suppressed RhoA activation, downregulated ROCK, prevented myosin light chain (MLC) DSS-treated mice, indicating effect attributed suppression RhoA/ROCK signaling pathways. These findings confirm active component from UC.